The medium was exchanged with fresh medium lacking phenol red.
However, allograft bone lacks osteogenic capacity as it does not contain living bone cells; therefore it is not considered to be a good source for osteogenic cells.
Cells were cultured using basic medium or osteogenic medium as extract basis.
Furthermore, this stimulatory effect was observed not only in osteogenic medium, but also in normal growth medium without osteogenic reagents such as l-ascorbic acid, glycerophosphate and dexamethasone.
All effects of GAG derivatives on TNAP activity were found in basic medium (without osteogenic supplements) and osteogenic medium (DMEM supplemented with β-glycerophosphate, ascorbate, dexamethasone), as well (see supplementary Fig. S3). Figure 6: Impact of GAG derivatives on TNAP activity.
In the scaffolds cultured in control medium, or osteogenic medium or in scaffolds switched to osteogenic medium for 1 week, mineralised tissue was observed both histologically and by μCT, but this lacked any significant structural organisation or surrounding marrow.
Cubic scaffolds were seeded with hMSC and either cultured in stem cell medium or osteogenic stimulation medium.
I-collagen protein staining in the cytoplasm was stronger in the experimental groups (strain + osteogenic medium) at 7 days than in the control groups (general medium or osteogenic medium).
We found that I-collagen protein staining in the cytoplasm was stronger in the experimental groups (strain + osteogenic medium) at 7 days than in the control groups (general medium or osteogenic medium).
BM = Basal medium, OS = Osteogenic medium.
