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Increased proliferation of ZR-75-1 cells treated with EGFR (0.01-10 ng ml-1) was only observed in serum-free medium lacking insulin and oestradiol.
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Medium lacking serum was termed 'basal medium' (BM), while medium containing 10% FBS was termed as "full medium" (FM).
Nevertheless, the finding that knockdown of Egr1 enhanced differentiation in medium lacking IBMX, but not dexamethasone or insulin, suggests that under normal differentiation conditions IBMX may act in part by inhibiting Egr1 action.
The medium was exchanged with fresh medium lacking phenol red.
Leu+ transformants were selected on SC medium (synthetic complete medium) lacking leucine (SC-Leu).
Transformed cells were plated onto complete SD medium lacking uracil.
Transformed cells were selected on medium lacking leucine.
Subconfluent cells were grown overnight in medium lacking serum.
At time point 0, the medium was changed to synthetic medium lacking glucose (red curves).
After 6 hours, the transfection medium was replaced with fresh medium lacking siRNA.
For serum starvation, the supplemented medium was replaced with medium lacking serum.
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