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Comparable untreated control cultures were maintained in identical medium lacking chloroquine with similar media changes.
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Medium lacking serum was termed 'basal medium' (BM), while medium containing 10% FBS was termed as "full medium" (FM).
Leu+ transformants were selected on SC medium (synthetic complete medium) lacking leucine (SC-Leu).
Cells with p316CupNGMC were grown in dextrose medium lacking uracil and diluted to OD600 nm = 0.05 into the same medium and into identical medium with 25 µM CuSO4.
Culture of transformants was performed in liquid selective medium (lacking uracil).
Transformants were selected on SD medium lacking uracil and screened by PCR.
Thus, static biofilm assays were repeated using medium lacking NaCl supplementation.
Serum starvation was carried out by incubating cells in their corresponding medium lacking fetal bovine serum.
Interactions were selected in diploid cells on SC medium lacking adenine and histidine.
TSC2−/−ASM and TSC2-transfected cells grew at a comparable rate in the medium lacking EGF.
In brief, peritoneal neutrophils from TGA-treated mice were washed and resuspended in medium lacking antibiotics.
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