Like plants, C. reinhardtii has a cell wall and can grow in a medium lacking carbon and energy sources when illuminated [87].
Firstly, we have noticed that the cells with defects in the RecBCD enzyme die faster at 4°C under growing conditions (e.g., in ABM) than under non-growing (e.g., in minimal growth medium lacking carbon source) conditions (unpublished observation).
After washing the mycelia with the standard minimal medium lacking carbon sources, the mycelia was suspended in a fresh medium of the same composition as used for the pre-culture.
A. nidulans microarrays provided by the Pathogen Functional Genomics Resource Center (PFGRC) were used to compare transcript levels in an xprG + strain and an xprGΔ null strain after transfer to medium containing glucose as a carbon source or medium lacking a carbon source (carbon starvation) for 16 h.
The cells were harvested by centrifugation (4,000 × g, 10 min) and washed twice with CGXII minimal medium lacking the carbon source.
While conidiation does not normally occur under these conditions, transfer to medium lacking a carbon source does induce conidiation in submerged cultures.
In the wild-type strain very low levels of sterigmatocystin were detected after 24 h growth in medium containing glucose and much higher levels after transfer, for 24 h, to medium lacking a carbon source.
After pre-cultivation, the mycelium of H. jecorina was transferred to medium lacking a carbon source (de-repressing conditions) or to media containing 1% (w/v) glucose, glycerol (repressing conditions), D-xylose or xylan or 1.5 mM sophorose or xylobiose (inducing conditions).
The consortia and single strains were separately cultured until they reached stationary phase, and the liquid cultures were then centrifuged and washed three times with PCS medium (lacking calcium carbonate and a carbon source).
We incubated C. glabrata wild type, atg11Δ, pex3Δ atg17Δ, atg17Δ, pex3Δ and17Δ atg11Δg11Δ atg17Δ mutant cells in medium lacking nitrogen and carbon sources at pH 3.5 at 37°C for 24 h.
The transcriptional control of meiosis in budding yeast is of special interest, since it is composed of several transcriptional waves, controlled by different transcription factors, and has therefore been studied extensively [ 3] In yeast, meiosis initiates in diploid cells upon exposure to medium lacking a fermentable carbon source and nitrogen.
