Sentence examples for medium lacking ammonium from inspiring English sources

Experiments were also conducted at different steady state concentration of ammonium sulphate with cells precultured in a medium lacking ammonium sulphate.

Plant residues and Frankia vesicle clusters collected through filtration were mixed in 100-ml flasks with 40 ml of modified BAP medium [ 28] lacking ammonium chloride (BAP-).

For gene expression, a single yeast colony was picked to inoculate a starting culture which contained 30 mL SC minimal medium lacking leucine (6.7 g L−1 yeast nitrogen base without amino acids, but with ammonium sulfate).

Pexophagy was induced by transferring cells to starvation medium lacking a nitrogen source (SD-N; 0.17% yeast nitrogen base without amino acids and ammonium sulphate, 2% glucose) (Hutchins et al, 1999; Manjithaya et al, 2010a).

The medium was exchanged with fresh medium lacking phenol red.

Leu+ transformants were selected on SC medium (synthetic complete medium) lacking leucine (SC-Leu).

Optimal induction of amtB occurred in response to a switch from mid-log growth in Evans containing 30 mM ammonium chloride to Evans medium completely lacking a nitrogen source for a 30 min period.

The functionality of the transporter fusions was tested by complementation of ammonium uptake deficiency in a yeast mutant lacking endogenous ammonium transporters (Marini et al., 1997).

Since the Δmep1,2,3 yeast strain lacks endogenous ammonium transporters, ammonium uptake in these cells depends on episomally expressed transporters.

Briefly, 100-ml liquid cultures were grown to an OD750 of 0.5 in BG-11(NH4) medium, which lacked sodium nitrate and contained 2.5 mM ammonium chloride and 5 mM MOPS (pH 8.0).

Following electroporation cells were plated onto selection medium-lacking Histidine.