Sentence examples for medium lacking agar from inspiring English sources

Larvae of the desired genotype were selected at 56 64 h AEL and transferred into vials of cornmeal medium lacking agar.

Particularly, about 2 g of stem derived callus, grown in solid medium supplemented with NAA 2 mg/l were transferred into 50 ml of the same medium lacking agar, and used for establishing cell cultures.

About 2 g of white and friable callus, grown in solid medium supplemented with 2,4-D 0.5 mg/l, 2,4-D 1 mg/l and 2,4-D 1 mg/l plus BA 0.5 mg/l, were transferred to 50 ml of the same medium lacking agar.

P. pastoris transformants were selected by the ability to grow on minimal agar medium lacking histidine due to the presence of the histidinol dehydrogenase gene (HIS4) in the transforming vector.

Complementation was evaluated based on cell growth on SC agar medium lacking AAAs (SC-AAA), since TKLs are involved in AAA biosynthesis via effects on the supply of erythrose-4-phosphate.

Colonies were grown on selective agar medium lacking tryptophan and leucine (SD-T-L) to confirm the presence of both expression plasmids (Figure 2A).

Interactions were examined on agar plates with SD medium lacking leucine, tryptophan, and histidine (KUWLH), with or without 1 mM 3-AT.

Transformed yeasts were grown on agar plates with SD medium (synthetic minimal medium) lacking leucine and tryptophan.

We grew transformed yeasts on agar plates with SD medium (synthetic minimal medium) lacking leucine and tryptophan; interactions were examined on plates with SD medium lacking leucine, tryptophan, and histidine, with or without 3-AT.

After selection for the presence of the three plasmids, co-transformed yeast strains were grown overnight resuspended in top agar (0,7% in water) and spread on solid medium lacking leucine, tryptophan, uracil and adenine. 1 μg of rapamycin were deposed on Whatman paper discs, on the surface of the agar, and cells were incubated at 30°C for 5 days.

After transformation with pUA12, pUA13, pUA14, pUA15, and pUA16 plasmids, cells were grown in minimal medium lacking uridine (MM-uri) (0.67% yeast nitrogen base without amino acids, 2% glucose, 2% agar and 100µg/ml of the required amino acids).