Sentence examples for medium lacking acetate from inspiring English sources

Collected cells were maintained in TP medium (TAP medium lacking acetate) in light under 3% CO2 with shaking at 100 RPM.

The min1 mutant possesses a miniature eyespot characterized by disorganized and nonmembrane-apposed pigment granules in the chloroplast stroma when grown in medium lacking acetate as a carbon source (Lamb et al. 1999; Boyd et al. 2011a).

min1 mutant cells assemble a miniature, disorganized eyespot when grown in medium lacking acetate, but min1 cells grown in acetate-containing medium assemble eyespots that are more organized and closer in morphology to wild-type (Lamb et al. 1999; Mittelmeier et al. 2008).

When viewed by electron microscopy, the pigment granule spot in min1 cells grown in medium lacking acetate as a carbon source is a disorganized aggregation in the chloroplast stroma that lacks apposition to the chloroplast envelope and plasma membranes (Lamb et al. 1999).

To investigate the effects of acetate on the growth of both wild-type and mutant cultures of C. reinhardtii and Synechococcus sp. PCC 7002, cultures were grown on TAP medium containing acetate and on a modified form of TAP medium that lacked acetate (termed co-culture medium).

A mutant strain of Clostridium acetobutylicum lacking acetate kinase activity is expected to have reduced acetate and acetone production compared to the wild type.

Liquid cultures were grown in modified Sager and Granick medium I with added Hutner's trace elements (R medium) or without acetate (M medium), or in M medium lacking nitrogen (M-N medium) (Harris 1989).

The plasmid libraries were used to transform a ura3 auxotrophic L. kluyveri strain FM628 using a standard Lithium Acetate protocol [ 55] and plated on medium lacking uracil to select for ARS function.

Yeast were transformed using lithium acetate, and transformants were isolated after growth on solid selective medium (lacking histidine).

Yeast cells were transformed by the lithium acetate method [42] and transformants were selected on synthetic complete medium lacking uracil (Ura-d/o).

Yeast transformations were performed using the standard lithium acetate method (Gietz et al. 1995) and selected synthetic complete medium lacking uracil (SCM URA−).