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Hepatocytes were cultured using CS-C comedium medium kit (Cell Systems, Kirkland, WA, USA).
CFU-Hill were cultured with the EndoCult® Liquid Medium kit (StemCell Technologies, Vancouver, BC, Canada) according to the manufacturer's instructions.
The assay was performed by using the EndoCult Liquid Medium Kit® (StemCell Technologies, Vancouver, BC, Canada) using the manufacturer's protocol.
At the GP's office, a midstream urine sample was collected after perineal cleansing, using the BD Vacutainer® urine collection and transport medium kit.
The cells were cultured in the recommended medium by the manufactures (FGM-2 Bullet kit, Fibroblast Growth Medium Kit, and Fibroblast Medium, respectively).
When the cell reached 80% confluence, they were passaged with the use of a passage reagent group, supplied with the complete medium kit (Cell systems).
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A major retailer having 8 small kits 3 medium kits and 13 large kits 1 major incident kit (foil blankets large dressings should a crash occur in car park) 3 plaster kits 8 Body Fluid kits and 5 burns kits.
Nasopharyngeal (NP) swabs were collected from all enrolled cases and placed in cryovials containing virus transport medium (Copan kit, Italy).
MCF10A cells were maintained in Mammary Epithelium Cell Growth Medium bullet kit (Clonetics, Lonza, MD, USA), containing mammary epithelium basal medium supplemented with bovine pituitary extract, human epidermal growth factor, hydrocortisone and GA-1000 (Gentamicin Sulfate and Amphotericin-B).
Human colon cancer HCT116, prostate cancer PC3, LnCap cells and benign prostate PNT2C2 cells were cultured in RPMI 1640 (BioWittaker, Fontenay sous-bois, France) and PrEC cells in medium Bullet kit (Lonza, Walkersville, USA).
Collect, process and sequence technical controls from each batch of sample collection/storage medium, each extraction kit, and each PCR kit concurrently with the environmental samples of interest.
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