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When the conditioned medium is transferred to nonirradiated cells, it also precludes their colony formation.
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Conditioned medium was transferred to naïve cells, and viral p24 protein in the culture supernatant was measured by ELISA.
The flasks containing the cells in fresh medium were transferred aseptically to the bioreactor to start new batch fermentation.
The mycelium stored on PDA medium was transferred to new PDA medium in 9-cm diameter Petri dish and incubated at 30°C for 5 days.
After 70 h, each medium was transferred to a 500 mL screw-cap flask and centrifuged at 3000 rpm for 20 minutes.
After incubation for 2 h at 37°C, 100 μl medium was transferred from each well to a new black 96-well plate.
This medium was transferred into sterilized Petri dishes in a laminar air flow chamber (Microfilt Laminar Flow Ultra Clean Air Unit, Mumbai, India).
Two-step culture technique was employed and the appropriate amount of cells grown in YP medium was transferred to nitrogen free fermentation medium at different pH ranging between 5.0, 5.5, 6.0, 6.5 and 7.0.
(b) Radicle emergence was recorded and data are means ± SD (n = 30 35 plants) (c) One-week-old seedlings grown on standard medium were transferred to culture plates with concentrations of mannitol (0 300 mM) for 7 days.
A 15-ml volume of each sample from each of the medium was transferred to a 50-ml tube and incubated at 25 °C with shaking at 100 rpm for 48 h.
When the 30Kc6-expressing cells cultivated in the serum-containing medium were transferred directly to commercially available serum-free media, 30Kc6 expression increased the viable cell density by four-fold through inhibiting serum deprivation-induced apoptosis.
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