Sentence examples for medium is subsequently from inspiring English sources
Virus-containing medium is subsequently harvested at ~24 and 48 h thereafter.
The medium was subsequently refreshed every other day.
M9-based culture medium was subsequently optimized for the enzyme production by orthogonal experimental design in shake flasks.
The culture medium was subsequently discarded and replaced with 150 μL of DMSO.
Fresh medium was subsequently added, and the cells were shifted to 37 °C to allow synchronous infection.
The filtered TSB medium was subsequently incubated at 30°C for 14 days.
The culture medium was subsequently replaced with specific inductive media to determine the adipogenic, osteogenic, and chondrogenic differentiation potential as described previously (Marchal et al. 2012).
Whole medium was subsequently replaced weekly.
The medium was subsequently changed every other day.
The medium was subsequently aspirated and cells were quickly washed twice with ice-cold Hank's balanced salt solution (HBSS) and harvested with tripsin-EDTA.
About 106 pfu of virus in 270 μl neuron medium was subsequently added to axonal compartment and incubated for 1 hour to allow virus entry.
