Sentence examples for medium is removed from inspiring English sources

After incubation, the SPMNPs containing medium is removed, washed twice with fresh medium and incubated for the chase period (0 24 h) with fresh medium.

Briefly, after 72 h of treatment, the medium is removed, 100 μL of 0.5% of crystal violet in 50% methanol is added to each well and incubated for 20 min at room temperature and subsequently excess dye is washed out gently by distilled water.

When fly cohorts are maintained in population cages of the design that we used, eggs laid away from the food medium fail to develop successfully, while the food medium is removed sufficiently often that development cannot be completed when the eggs are laid there.

The medium is removed and 1 ml medium containing 16 µg polybrene (Sigma) and 1 ml of viral supernatant are added.

After treatments, culture medium was removed and washed with PBS.

Then the medium was removed and the substrates were rinsed with PBS three times.

One hour after transfection, the medium was removed and cells were washed with PBS.

Then the medium was removed carefully, and 100 μl DMSO was added to dissolve formazan crystals.

The inorganic carbon of the fresh medium was removed by acidification and stripping with N2.

Every 2 3 days, medium was removed, cells were washed with PBS (Gibco) and fresh medium was added.

Then the growth medium was removed from cells and replaced with 3 mL of complete growth medium.