This was repeated 2 or 3 times, until the medium was clear.
After two days of growth in liquid medium containing either sucrose, carboxymethylcellulose (CMC, a soluble form of cellulose), or SigmaCell (SMC, insoluble cellulose powder) as the sole carbon source, spent Teredinibacter turnerae fermentation medium was cleared of cells by centrifugation and used for proteomic analysis.
Briefly, total culture medium was cleared by centrifugation.
Conditioned medium was cleared of cells and debris by centrifugation at 4000 r.p.m. for 10 min.
Briefly, the culture medium was cleared via centrifugation at 4,000 g for 10 min at 4°C.
Culture medium was cleared by centrifugation and concentrated using Ultracel-3K Amicon Ultra centrifugal tubes (Merck Millipore Ltd ,Germany).
Collected medium was cleared by centrifugation, filtered, and used undiluted on target cells for 2 hours prior to collection of cell lysates.
To separate microvesicles and soluble proteins, the medium was cleared by centrifugation and filtering and subjected to ultracentrifugation at 100000 g for 1 h at 4°C.
The culture medium was cleared by centrifugation at 2,000 × g for 10 min. The culture medium of WT AcMNPV-infected cells was prepared in the same manner and used as a SEAP-negative control.
From the observation of the trends at all mediums, it is clear that the increase of erosion-corrosion rate is very high due to the change in the flow regime from laminar to turbulent, especially at seawater with 9 g/l of sand at turbulent flow.
