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In this article, the lateral deflection of piles constructed in a multilayer soil medium is assessed.
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In addition, alkaline phosphatase activities of cells cultured on the scaffolds in conditioned medium were assessed.
Additionally, the effect of the presence or absence of insulin-like growth factor-1 (IGF-1) in the culture medium was assessed.
Moreover, the morphological and stability features of these colloidal ZnCdS conjugates in aqueous medium were assessed by DLS analysis.
The medium was assessed for cellular secretion of IL-8 using an IL-8 sandwich ELISA DuoSet kit (R&D Systems Inc., Minneapolis, MN, USA) as per manufacturer's instructions.
To circumvent this problem, direct C. albicans infection of the worms by co-inoculation in assay wells containing the liquid medium was assessed.
Muc5AC levels in the culture medium were assessed by ELISA using commercially available human anti-Muc5AC ELISA essentially as described by the manufacturer (Cosmo Bio USA; Carlsbad, CA).
The activity of MMP2 and MMP9 in the conditioned medium was assessed by zymography.
Total PAI-1 protein secretion in culture medium was assessed by enzyme-linked immunosorbent assay.
Measurement of lactate dehydrogenase (LDH) released to the medium was assessed as a cell death indicator.
Growth in liquid medium was assessed by measuring optical density at 580 nm with a spectrophotometer.
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