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Each tube contained 5 ml of LB medium inoculated with 106 bacteria per ml.
Dried milled herbage samples were incubated at 39 °C in a buffered medium inoculated with rumen fluid obtained from fistulated steers.
Optimized conditions employed 4 mL of antibiotic 1 medium inoculated with 1% of Staphylococcus aureus suspension, and linezolid in concentrations from 25too 100 µg mL−1.
b Chromatogram of a 3-day-old culture medium inoculated with strain CS-1, mainly containing oxalic, tartaric, and citric acid.
Flask with 100 mL of autoclaved production medium inoculated with 1 mL of culture was incubated in rotary shaker at 200 rpm at 37 °C for 48 h.
Plastic wells were filled with a liquid medium, inoculated with 300 μL of 0.5 McFarland microbial suspensions and incubated for 72 h at 30°C.
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After sterilization, the fermentation medium was inoculated with 5% (v/v) seed medium.
The sterilized medium was inoculated with two 5 mm mycelial plugs, and a non-inoculated medium was used as the control.
After autoclaving and cooling, the food waste medium was inoculated with 40 mL stage-two culture developed in P2 medium and fermentation was initiated.
Then the medium was inoculated with 40 mL of stage-two culture developed in P2 medium, followed by fermentation at 35 °C in the anaerobic chamber.
For sterigmatocystin assays, flasks containing 50 mL of Aspergillus minimal medium were inoculated with 3 x 10 conidia scraped from cultures grown on complete medium containing 2.2% agar.
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