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For neurobasal medium induction protocol, clear morphology changes begin as soon as two days after induction and became more prominent around 4-5 days.
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We show that PfTrx-L2 is produced at high levels (up to 100 mg from a 100 ml starting culture using a multi-cycle induction protocol) and secreted into the culture medium as a highly enriched (>70% pure), non-glycosylated polypeptide that can be purified to homogeneity in a single step.
The induction protocol in this paper used serum-free culture medium, which we demonstrated to be sufficient to induce the morphological changes in MSC formerly believed to be neurons.
Cell growth of each strain in a PBS-diluted Sabouraud capsule induction medium, with the exception of the cap59Δ isolate, resulted in robust capsule formation, as shown for strain JEC21 (Fig. 4E), validating the effectiveness of the capsule induction protocol.
Before and after the induction protocol application, the EPSCs were evoked each 20 s.
This tolerance induction protocol enriches for alloantigen reactive regulatory T cells (Treg).
In a second set of experiments, a prolonged amyloid induction protocol was used.
In the modified medium, induction is also obtained after addition of specific copper chelators, like TETA.
Repeated Induction protocol.
We tried two induction methods: retinoid acid (RA) induction and neurobasal medium induction.
Mice were immunized according to the reduced collagen induction protocol.
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