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The differentiation rate of ASCs is 38.6 ± 11.2% for neurobasal medium induction method and 45.5 ± 8.3% for RA induction method.
Both conditions can induce UC-MSCs to differentiate into neuron-like cells as well but with a much lower efficiency (22.3 ± 4.8% for neurobasal medium induction method and 18.4 ± 5.6% for RA induction method (P < 0.01)).
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We tried two induction methods: retinoid acid (RA) induction and neurobasal medium induction.
The central interest of many works is to optimize production conditions such as shaking speed, medium, induction temperature, IPTG concentration, but the optimization of the protein extraction method remains a major challenge for a high percentage of the produced protein.
Interestingly, alone DMEM/F12 medium can induce ASCs to transform to neuron-like cells, similar to RA induction method (data not shown).
Interestingly, alone DMEM/F12 medium can induce ASCs to transform to a neuron-like cell, similar to RA induction method.
Using this method, we measured intervals between consecutive productions of RNA molecules under the control of lar, under weak and medium induction, which have not been previously measured.
Effects of medium induction on the cytokinin synthesis pathway and the brassinolide synthesis and transduction pathways.
Then iN cells were seeded onto cortical neurons bed and maintained in Mature Medium (Induction Medium with 0.5 mM dbcAMP, 10 ng ml−1 BDNF, 10 ng ml−1 NT3).
Effects of medium induction on the proteins and metabolites of the jasmonic acid (a) and ethylene (b) synthesis pathways.
We use the induction method on m.
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