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While both "DXM" medium and "BMP-7 + DXM" medium induced the DPSCs to odontoblast-like cells, the "BMP-7 + DXM" medium had greater inducing capacity than the "DXM" medium.
M2-conditioned medium induced the proliferation of VSMCs (Fig. 1B) and of adventitial fibroblasts (data not shown).
The protective sucrose plus atrazine medium induced the expression of these transcription factors whereas the atrazine treatment had little effect.
Growing the histidine and tryptophan positive clones in the galactose medium induced the expression of Cre recombinase.
As indicated in Fig. 3a, 5C medium induced the direct conversion of astrocytes to TuJ+ cells within 14 days, while no Nestin+ cells were identified.
5C medium induced the majority of MEFs to undergo morphological changes including the shrinking of cell and nuclear sizes, the formation and elongation of cell surface protrusions at multiple points, and the adoption of typical neuronal morphology (Fig. 1e).
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When a gene of interest is subsequently positioned under the control of the nisin-inducible promoter PnisA in a plasmid such as pNZ8148, the addition of sub-inhibitory amounts of nisin (0.1 5 ng/ml) to the culture medium induces the expression and the production of the corresponding protein (for review, see [13]).
Future work will focus on the temporal variations of the medium induced by the operations conducted at the geothermal sites (drilling, injection/production tests, etc).
According to the swelling and electro-mechanical testing results, the electrically driven deformation of the hydrogels was interpreted as a dynamic osmotic equilibrium effect taking place at the interface between the polymer and the surrounding medium, induced by the free ionic species migration throughout the polymer.
In addition, the simultaneous and real-time recording of neuronal activity and the environmental temperature revealed that the neuronal modulation was accompanied by a 0.5 1 °C rise in the temperature of the culture medium induced by the heat generation of exposure coils.
One-hour incubation of MII oocytes in PJ-34-containing medium induced breakage of the bipolar structure of spindle bundle (untreated; 0/5 (0%), PJ-34 treated; 6/11 (55%)) with intact cytoplasmic asters (Figure 3C, D).
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