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In our experiments, MIT addition to the incubation medium induced a significant increase in glucose-induced insulin release, either from fresh or precultured isolated islets.
However, the full adipocyte differentiation medium induced a much more robust adipogenesis in these cells.
Injury conditioned medium induced a more robust calcium response than the No Injury control (Figure 3A; first two rows).
The new culture medium induced a slight morphology change, as compared to the serum condition (Figure 9A).
In βhigh-cells, increasing the glucose concentration from 5.5 mM to 8.3 mM and to 16.7 mM in the perifusion medium induced a marked increase in insulin release (Fig. 2A).
Addition of the cholesterol crystals to culture medium induced a dose-dependent increase in the cholesteryl ester content of primary human monocyte-derived macrophages and PMA-differentiated THP-1 macrophages (Fig. 1A).
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The addition of PD-98059 in the culture medium induced an increase of miR-21 expression in a dose-dependent manner (Figure 1C).
Preincubation of intact segments with ouabain (100 µM) for 30 min in K+-free medium induced an increase in vascular tone in the aortas from both groups, but the increase was smaller in the last concentration of segments from lead-treated rats (Figure 6 A).
The addition of NPs to fibroblasts culture medium induced an enhancement of FAK activation in early times (0.5 and 2 hours) and in dose depending manner and 4(c)).
As expected, the Cl− substitution by gluconate in the apical medium induced an increase in the Isc to 16.0 μA per cm due to the diffusional flux of Cl− from the basolateral compartment to the apical one without changes in the transepithelial resistance.
Results show that the addition of the essential oil of the plant to the medium induces a diminish in the rate of corrosion and augmentation in the inhibitory efficiency of the oil.
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