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After passage 3, cell growth performance in glutamine-free medium increased significantly.
Likewise, the concentration of IGFBP-1 protein secreted into the culture medium increased significantly after a 72 h cultivation period.
Post hoc Tukey analysis indicated the incorporation of the FhrAPS to the culture medium increased significantly the production of Duddingtonia spores, whereas no effect on Mucor was recorded (Table 2).
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EN-RAGE protein was detected in the cultured medium and increased significantly by the addition of IL-6.
Comparing uncoated sample with BG-coated samples, it is obvious that in the presence of bioactive glasses, Si concentration in culture medium has increased significantly (Fig. 5a).
In the first set of experiments, the amount of HA in the medium was increased significantly 3.66 fold by the addition of 5 mM GlcN-HCl when compared to the control condition (Fig. 1).
The contents of all studied parameters in N. oculata grown on the optimized medium after10 days increased significantly (P ⩽ 0.1) than those on control with low concentration of PO4 (2.5 g l−1) and with high concentration of NO3 (112.5 g l−1) except for cell numbers and DPPH.
By 2012 2013, the number of large and medium reservoirs had increased significantly since 1958.
The amount of added phosphoric acid to adjust the mediums pH level increased significantly after 34 h of cultivation.
The use of this medium increased growth significantly, as the OD600 was able to reach nearly 10 after just 24 h, compared to the previous high of 4.5.
At the second day of treatment, at 0.5% DMSO in culture medium, cell multiplication was increased significantly whereby at 2% a significant attenuation was observed (Fig. 11A).
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