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Nonetheless, the treatment of conditioned medium in vitro cannot fully recreate the condition of cell therapy in vivo where multiple intercellular activities were involved.
Canine bone marrow stromal cells were cultured in a PLGA collagen hybrid mesh in osteogenic medium in vitro and laminated to construct an osteo layer.
iNANOG cells were cultured in E8 or 2il/FK medium with or without Dox, then injected into E3.5 mouse blastocysts and cultured to E4.5 in KSOM: 2il/FK = 1 1 medium in vitro.
Compared with the rapid release of free Ats in medium in vitro, Ats entrapped in the core of BSA NPs was slowly diffused from the interior of NPs into the medium and showed a smooth and sustained release pattern, owing to the continuous degradation of BSA.
Also, cell-bound albumin, present on the surface of lymphocytes and macrophages, was found to originate either from the tissue culture medium in vitro or from serum in vivo [17].
An example of distributional bias of genes induced under a particular condition is Vibrio cholerae which, when grown in an in vivo mouse intestinal model, shows a greater number of induced genes on the smaller of two replicons compared to growth on rich laboratory medium in vitro [61].
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As soon as they enter a biological medium (cell culture medium for in vitro, blood or plasma for in vivo studies), nanoparticles, in most cases, see their surface covered by biomolecules, especially proteins.
The plantlets with 3 4 roots about 2.5-3 cm long were then transferred to DE basal medium, an in vitro mycorrhizal induction medium in orchids [ 27].
and washed with Quinn's Advantage Blastocyst Medium (SAGE In-Vitro Fertilization, Inc. A Cooper Surgical Company) with 3% KSR (Gibco) added.
The oocyte denudation was performed by a brief exposure to 40 IU/mL hyaluronidase solution in fertilization medium (Sage In-Vitro Fertilization, Inc., Trumbull, CT, USA), followed by mechanical removal of all the remaining cumulus and corona cells with the use of plastic pipettes of defined diameters (denuding pipette; COOK Ireland Ltd., Limerick, Ireland).
In most of the published data either minimum essential medium (MEM), Dulbecco's modified eagles medium (DMEM) or F99: Hams F 12 is used as the basal medium for in vitro culture of CEC [ 11].
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