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RAW264.7 were purchased from China Center for Type Culture Collection (CCTCC) and cultured with high glucose DMEM medium supplemented with 10% FBS, 1% antibiotics for cell expansion and replaced with low serum (1% FBS) medium in preparation for further analysis.
Then, it is interesting to examine the interaction of ethanol as impregnation medium in preparation of Ni/MgAl2O4 CeO2 catalysts.
Twenty-four hours prior to the NCCM-related experiments the plated bovine NP cells were extensively washed with basal medium and then cultured in basal medium in preparation for the NCCM experiments.
For the 2% NCCM experiments, we initially thawed bovine NP cells and expanded them using complete medium, then washed them three times with 2% FBS supplemented basal medium in preparation for these experiments.
In so doing, we were able to capture single cells (e.g., cell isolate #9-2i; Figure 12) to which the mCherry-labeled nanobody was bound and culture them on solid medium in preparation for taxonomic classification based on DNA sequencing of their 18S ribosomal RNA genes (described below).
Moisten the medium in preparation for sowing the seeds.
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In Mexico, officials closed 18 ports on the Gulf of Mexico to small- and medium-sized craft in preparation for the storm.
Pipette 200 μL of the freshly prepared elution medium in the same sample preparation vial.
The nutrients used in medium preparation are not recycled during the MEOR process; hence, the cost involved would affect the selection of optimized condition.
Ultra-pure water used in medium preparation and assays was processed with a Milli-Q Gradient system from EMD Millipore (ON, Canada).
Tumor tissue was postfixed for 10 minutes in NBF at room temperature prior to cryoprotection overnight at 4°C in 30% sucrose-PBS, embedding in OCT medium and preparation of 10 μm sections by cryostat.
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