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Experiments were conducted with the fungal biomass and with a liquid medium in parallel.
To approach these questions we measured cAMP levels at 37°C after 1 pmol/l E2 stimulation, in cells and in their extracellular medium in parallel.
Transcript levels of genes encoding enzymes involved in SAM metabolism were higher in cells transferred to induction medium compared to those transferred to control medium, in parallel with the observed increase in lignin.
To assay expression of mesodermal marker Xbra Animal Caps AC), explants were removed from injected embryos at stage 8 9 using forceps on agarose coated dishes in 1× Barth's medium and cultured in 0.5× Barth's medium in parallel with control embryos and stored at −80°C before gene expression analysis.
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Placental histocultures (18 20 fragments) were exposed to virus stocks containing 15 40×106 genome equivalents in a final volume of 2 ml of washing medium, and in parallel, to washing medium alone (mock infection).
Conditioned medium was collected from the BACE1 and control cells grown to equal confluence, and the medium combined in parallel for HEK and HeLa cells.
Thus, during long term culture the growing tissue is exposed to always fresh medium preventing in parallel an un-physiological accumulation of metabolic products and an overshoot of paracrine factors.
Instead, since protection by Ly294002 in nutrient-rich medium occurred in parallel with inactivation of the mTOR cascade, these findings reinforce the idea that 1) mTOR signaling is absolutely critical for cell death in this experimental context, and 2) that beneficial effect of mTOR occurs also in the context of nearly complete AkT inhibition.
Therefore, the contribution of various competing components was calculated by assuming the scavenging of ABTS+ by antioxidative species produced by the culture and the MRS medium in a parallel first-order reaction.
Length here equals genuine suspense in a movie that is still the cinema's most delightful statement on the way we have made a medium that runs in parallel with life and may yet take it over.
As a cell growth inhibition control, growth curves in which 200 µM rotenone and 0.5 µM antimycin were added to the culture medium were run in parallel for all experiments.
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