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After counterstaining in haematoxylin, slides were mounted in gelatine mounting medium (in-house).
To design the ORC unit and select the working medium, an "in-house" optimization tool has been developed.
An analysis of CD3+ cells from all healthy TIL cultures from patients #M27 - M45 (cultured in medium containing "in-house" human plasma) revealed an average of 37% +/− 28% CD4+ T cells (median 33 % and 52% +/− 30% CD8+ T cells (median 52%).
We also noted that functional assays of TILs that had been cultured in medium containing "in-house -generated human plasma yin-house -generatedoportin-house -generatedve cultures compared withumans that had been cultured in medium containing commercially available human serum (data not shown).
NPS were collected into STGG transport medium (prepared in-house) and processed according to the WHO pneumococcal carriage detection protocol [ 13].
Transformed cells were recovered using 1 mL of SOC medium (prepared in house) and plated onto 22 cm × 22 cm agar plates (Genetix) containing 2xYT agar and 100 μg/μl Ampicillin.
This interaction was most pronounced for HzSNPV, which experienced a dramatic decline in biological activity when switched from its original Excell 401 medium to the in-house VPM medium.
The LJ-NRA medium was prepared in-house.
In conventional system, the culture medium is made in-house, blood culture bottles are incubated in a conventional incubator, and bacterial detection is made by direct visualization with or without regular sub-culture.
NPA specimens, in 1 ml viral transport medium (VTM, prepared in-house), were transported daily to the SMRU microbiology laboratory, which is located in the town of Mae Sot, approximately 50 km from Maela.
In the first step, 48 of 52 components of our in-house medium were divided into three groups according to their characteristics.
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