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Aliquots of culture were diluted with fresh medium in glass test tubes to obtain an OD600 = 0.19, and supplemented with 100, 20, 10, or 2 µg of ovorubin, respectively; sterile buffer was used as control.
Plantlets were then sub-cultured in vitro on solid Heller medium in glass tubes [ 73].
For sequencing of pooled samples, all 36 isolates were grown independently in 2 mL LB medium in glass tubes at 37°C, 180 rpm over night.
Liquid-air biofilms were grown in 2216E or SW-LB medinm in glass beakers and test tubes without shaking for an indicated time at 25 °C.
Briefly, moss tissues were grown at 25°C under the cycle of 14 h light (90 μmol m-2 s-1) and 10 h dark for one week on a thin layer of solid PpNO3 medium in glass bottom dishes.
The effects of H2O2 and catalase (Sigma-Aldrich) on the intrinsic H2O2 concentrations and flowering time were determined using plants grown on MS medium in glass bottles (5 cm high, 2 and 1.5 cm wide diametrically at top and bottom).
Similar(52)
From a lineup of liquid mediums in glass bottles, each of which had a different consistency and drying time, he selected a mixture of stand oil and balsam and poured a little of it into a small cup that was affixed to the palette.
After the INS-1 cells were loaded with 10 mM glucose and 5 µM Fluo-3/AM, amolopin samples were added into incubation medium in the glass bottom dishes.
The separated zygotes were individually isolated and placed in 500 µL AF-6 liquid medium in a glass depression (20-mm diameter) in Petri dishes.
For pre-culture, 4 mL of synthetic glucose medium in a glass tube were inoculated using cells from a YPD plate and incubated overnight at 200 rpm in an orbital shaker.
Since the volume of medium in the glass container was four times higher than that in the Petri dish, the results obtained were positive, since there was no indication that scaling up has a negative effect the growth and lipid production of this species of myxomycete.
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