Sentence examples for medium in flasks from inspiring English sources

Orchid seed is as fine as dust and must be sown on a sterilized nutrient medium in flasks.

After complete of the selection, medium in flasks containing attached transduced cells was replaced with fresh complete growth medium and changed every 3 ~ 4 days thereafter.

The medium in flasks taken out must be compensated with 9 K medium, and the evaporation of water in flasks was supplemented with distilled water.

To monitor the cellulase production and measure the hydrolytic activity of the cellulases produced, the Y. lipolytica[EGII] and [CBHII] transformants were cultured in YPD medium in flasks, and samples were taken daily.

Chlorella sorokiniana 7-11-05 [ 55] was obtained from the UTEX Culture Collection University off Texas, Austin, TX [ http://web.biosci.utexas.edu/utex/]) as strain UTEX1230; maintained on BG-11 agar plates [ 56] and grown photoautotrophically in BG-11 medium in flasks at 25°C, 70 μmol photons m-2 s-1, with a photoperiod of 16:8 h light:dark.

Metabolic flux ratio (METAFoR) analysis was performed for the T. reesei wild type (QM6a) and Δ cre1 (L161a) strains growing in minimal medium in flasks with fractional [U-C]glucose and on fractional [U-C]sorbitol with and without induction of cellulase gene expression by sophorose.

Among fatty acids tested, long chain saturated fatty acids, such as C18 (stearic acid) and C20 (arachidic acid), were found to function as effective inducers for the production of lipase, giving an activity level almost the same as that obtained in C2 medium in flask culture.

Calcium carbonate (CaCO3, 1 %  w/ v) was used to buffer the medium in flask cultures.

The conidia suspension, suspended in sterile deionized water with 0.5% Tween 80 (Sigma-Aldrich, America) at a concentration of 1 × 10 conidia/mL, was inoculated in a 100-mL liquid medium (in flask) and incubated in a shaker at 25°C and 200 rpm.

The cultures were incubated at 150 rpm on rotary shaker at 30 °C for 4 days in 50 mL Erlenmeyer flasks with 10%% of medium in each flask, and a flask without cell mass was used as controls.

The first cycle of repeated batch culture was run by transferring 20% (v/v) of seed culture (40 mL) into 160 mL fresh medium in Erlenmeyer flasks (500 mL) to make a final 200 mL culture medium in the flask and incubating at 30°C.