The initial AR80 concentration in the culture medium in each bottle was 1.0 × 106 cells/mL.
Afterwards, the medium in each well was then removed and 200 μL of DMSO was added to dissolve the internalized purple formazan crystals.
The total volume of medium in each tube, including X(Cr, Mn, Co, or Ni -modified goethite (FeOOH) aNi -modifieds 250 ml.
The inoculum was grown in three 500 mL shake flasks (100 mL medium in each flask) at 28.0 ± 1°C and 200 rpm overnight.
All steps were repeated the same way, but 100 μl of the composite saline extracts were added to 400 μl of medium in each well.
The conditioned medium in each well was replaced with 100 μL of fresh medium containing WST-8, and the cells were incubated for a further 2 h, before measurement of the absorbance at 440 nm.
The cultures were incubated at 150 rpm on rotary shaker at 30 °C for 4 days in 50 mL Erlenmeyer flasks with 10%% of medium in each flask, and a flask without cell mass was used as controls.
After removal of the medium in each well, 150 μl of DMSO was added to each well and the plates was shaken until all the formed formazan blue crystal inside the wells dissolved.
After incubation, culture medium in each well was discarded and replaced with 50 µL of DMSO.
The carrying capacity K represents the total volume of the medium in each culture.
Water was added to the surface of the growing medium in each module over a period of approximately 30 s.
