Sentence examples for medium in duplicate from inspiring English sources

The fermentations of the design were carried out in stirred flasks containing 200 mL of culture medium in duplicate.

Soil used for mycobacterial isolation was treated as described above, and each sample was inoculated onto the modified selective medium in duplicate for culture at 30 and 37 °C, respectively.

The metabolites from B. amyloliquefaciens N3-8 showed more broadened antimicrobial activity against B. pseudomallei and was therefore selected to grow in minimum medium in duplicate to observe the production of antimicrobial metabolites in time by the agar well diffusion method.

Briefly, 1 × 106 cells were seeded onto 60 mm cell culture plates in 2 ml completed medium in duplicate.

Cells SaOS-2 and SJSA-1 were seeded at 5 × 10 cells per insert in serum-free medium in duplicate.

We measured the testosterone secreted into the medium in duplicate by radioimmunoassay as previously described (Habert et al. 1991).

After 24 h the inoculum was prepared by transferring 5 mL of the pre-inoculum to a 50 mL screw-cap flask containing 45 mL of the growth medium described above (in duplicate).

The mixtures were then diluted 1000-fold in GTB mixed with overlay medium and plated in duplicate on agar enriched with trypticase soy broth (TSB).

For poliovirus titration, 10-fold serial dilutions (1∶10 to 1∶109) of the culture medium were added in duplicate to HeLa cells cultured in 48-well plates.

A morphine-specific ELISA kit (Immunalysis Corporation, USA) was used for determination of morphine present in culture medium (40 µl in duplicate; n = 6 or 7) or in the serum of patients (20 µl, in duplicates).

The extracts (20 μl/well) at varying concentrations, diluted in culture medium, were added in duplicate.