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Exact(6)
In controls, vehicle (sterile normal saline) was added to the culture medium in control group.
After 48 h of drug exposure, the medium in control and drug-containing wells was removed.
DMSO alone was added to the culture medium in control group ("Con-group").
We began the indicated treatments at day 10, the culture medium in control group also changed into serum free, and measured the TER at 1, 2, 3, 12, 24, 48, and 72 hours after exposure to 100 nM Tat.
Significantly more GAGs were found in cells plus medium in control and high glucose plus insulin treated cultures at 72 compared to 24 hours and to other groups at 72 hours.
When the ratio of GAGs in cells and medium was expressed as a percentage, there were more GAGs in cells than medium in control and insulin alone treated cultures, while in high glucose treated cultures with and without insulin the ratio was reversed.
Similar(54)
Histo- and/or immunohistochemical techniques for the detection of bone-specific markers were applied to cells grown in osteogenic differentiation medium and in control medium to assess the osteogenic differentiation of rBM-MSCs.
Control medium consisted of DMEM (high glucose) supplemented with 10% FBS, 100 U/ml penicillin, 100 μg/ml streptomycin and 0.25 μg/ml amphotericin B. Differentiation medium consisted in control medium supplemented with 10% FBS, 1 μM dexamethasone, 0.5 mM indomethacin, 10 μg/ml insulin and 100 mM 3-isobutyl-1-methylxanthine (all from Sigma).
Therefore, the ABE yield was 0.38 g/g, which was comparable to the yield of ABE fermentation with glucose medium in the control experiment.
There was a significant (P < 0.05) increase in the overall lactate production rate in response to the glucose concentration in the medium in the Control and PCOS groups while the increment was not significant in the Metformin group.
In addition, alkaline phosphatase (ALP) assay also showed the increment of ALP activity after 10 and 14 days of mononucleated cells cultured in osteoblast differentiation medium compared to cells cultured in control medium (proliferation medium).
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