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Collected supernatants were replaced with the same amount of fresh culture medium; half of the culture medium was replaced with fresh complete medium after 5 days.
After 6 days of feeding on the reduced nutrient medium, half of the wild-type larvae reached the third instar developmental stage.
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CR.pIX cells were infected with A/PR/8/34 without medium exchange, after a partial medium exchange (half of the culture), or after a complete medium exchange.
In the medium term, half of the value of our business is on the continent, and anything that makes it more expensive or difficult to operate will be negative.
On MG medium, over half of the essential genes were involved in amino acid (36.05%) and nucleotide metabolism (23.26%; Figure 2).
After one following washing step with the cultivation medium, one half of the cultures exposed to the indicated concentrations of BAP was stimulated with 1 μg/ml of lipopolysaccharide (LPS) for 4 hours.
Further medium changes of half of the total medium per well were performed on DIV 1, 2 and 3. Cells were fixed or lysed for further analysis on DIV 5.
For a partial medium exchange, only half of the culture volume was centrifuged, re-suspended in fresh medium and subsequently pooled with the remaining non-centrifuged culture.
On day 3 of culture, additional 10 ml medium was added, exchanging half of the medium on day 6.
After a few hours the cells settled and the inserts were transferred into spinner flasks (Techne Ltd) with 50 ml RPMI 1640 medium per insert and half of the medium was refreshed every third day.
To prevent nutrient limitation other than nitrate, all BBM medium salts (at half of the usual concentration) were also added each time nitrate was supplemented.
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