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Culture medium (half of volume) was replenished with fresh medium containing mIL-2 every 3 days.
Collected supernatants were replaced with the same amount of fresh culture medium; half of the culture medium was replaced with fresh complete medium after 5 days.
After 6 days of feeding on the reduced nutrient medium, half of the wild-type larvae reached the third instar developmental stage.
These neurospheres were individually subcultured in neurosphere medium; half of these neurospheres exhibited a capacity for self-renewal for three subsequent passages, with similar efficiencies for the irradiated mice and the controls (Fig 1H).
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This study demonstrated a significant lack of fit of a medium half face respirator across all race groups and both genders.
CR.pIX cells were infected with A/PR/8/34 without medium exchange, after a partial medium exchange (half of the culture), or after a complete medium exchange.
The medium half-life of 227Ac (21.77 years) makes it very convenient radioactive isotope in modeling the slow vertical mixing of oceanic waters.
In the medium term, half of the value of our business is on the continent, and anything that makes it more expensive or difficult to operate will be negative.
On MG medium, over half of the essential genes were involved in amino acid (36.05%) and nucleotide metabolism (23.26%; Figure 2).
After one following washing step with the cultivation medium, one half of the cultures exposed to the indicated concentrations of BAP was stimulated with 1 μg/ml of lipopolysaccharide (LPS) for 4 hours.
Further medium changes of half of the total medium per well were performed on DIV 1, 2 and 3. Cells were fixed or lysed for further analysis on DIV 5.
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