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Minimal medium growth experiments were carried out at 37°C, with absorbance measured (at A600) over time.
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Because no clear effects of αSyn or the other brain proteins could be observed in WT BY4742 cells grown in normal inducing medium, we performed growth experiments in the presence of DMSO.
Here we note that g and Tmax were separately estimated and fixed to be 0.47 ± 0.10 for the static culture and 0.54 ± 0.09 for the shaking culture per day, and (1.51 ± 0.02) × 10 and (1.22 ± 0.02) × 10 cells per flask of medium from the cell growth experiments, respectively (see 'Materials and methods', Figure 2 figure supplement 2 and Supplementary file 2).
Growth experiments in minimal medium (basal salts medium; BSM) with salt concentrations typical of sea water (an average of 3.5% NaCl) showed that while all Burkholderia species can replicate at this salt level, only Burkholderia gladioli grew reasonably well (Table 3).
The mineral salts medium used for isolation and growth experiments contained the following in 1 l of deionized water; 1.55 g K2HPO4, 0.85 g NaH2PO4, 0.5 g NH4Cl, 0.1 g MgSO4 · 7H2O and 0.1 ml trace solution described by Vishniac and Santer (1957).
For growth experiments, CGXII minimal medium (Eggeling and Reyes [2005]) was used for C. glutamicum.
In all growth experiments in liquid medium, the inoculum size was 1% (v/ v).
Glycerol was the carbon source provided in the medium for the below-freezing temperature growth experiments [ 8].
For growth experiments, the electron donors were added separately from the prepared stocks to the NBAF medium.
Cell growth experiments were performed in 96-well plates by seeding 2000 10000 cells in 100 µl growth medium.
The production of siderophores was examined by aerobic growth experiment on GASN medium [ 16].
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