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There was no statistically significant difference in the free intracellular Ca2+ between the low glucose condition and the medium glucose condition.
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IP3R-3 was detected in both the nucleus and cytoplasm in medium glucose conditions.
In fact, there was no statistical difference between the low and medium glucose conditions for all assays tested.
In low and medium glucose conditions, there was no detectible SERCA2 in the nuclear fraction, but in high glucose a clear band appeared.
In normal (medium) glucose conditions reported here, the distribution of the IP3R subtypes, RyR, and SERCA were similar to other studies using primary aortic SMCs [ 23], rabbit aortic SMCs [ 24], and cultured cell lines [ 25].
However, addition of exogenous fatty acids alone did not increase the concentration of TG in the medium under either glucose condition (Fig. 5 C ).
Therefore, only medium and high glucose conditions are reported in this study.
Figure 3B illustrates the altered responses to both vasopressin (time 0) and thapsigargin (TSG, arrow) from cells grown in medium and high glucose conditions.
After attachment, cells were cultured in the medium under different glucose conditions for 5 days with/without various treatments as noted above.
Under medium and low glucose conditions, the timing of the Ca2+ transient was synchronous between the cytoplasmic and nuclear compartments of the A7r5 cells.
Cells grown in medium and low glucose conditions (n = 74 and 97 cells, respectively) displayed 99% synchrony in the nuclear and cytoplasmic Ca2+ signals produced by vasopressin or thapsigargin.
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