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Positive signs show interaction as growth on selective medium from three independent experiments.
Conditioned medium from three independent experiments was pooled, aliquoted and stored at -20 °C until required.
Positive signs show interaction as growth on selective medium from three independent experiments (nd = not determined).
Twenty-four hours later, the medium from three wells was replaced with adipogenic (Gibco), osteogenic (Gibco) or chondrogenic (Gibco) medium, respectively.
This protocol outlines how to culture melanoma cell lines with conditioned medium from three stromal cell lines with or without the RAF inhibitor PLX4720 to analyze cell proliferation rates, as is described in Figure 2A.
Then a super-SILAC mix was developed by collecting conditioned medium from three representative different heavy stable isotope labeled cell lines, which served as an internal spike-in standard when being mixed with the conditioned medium of the tested cell lines for all samples accurate quantification.
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The incoming beam is split in two, and hits the medium from two sides.
Mr. Keïta, now in his 70's and with international stature, picked up the rudiments of the medium from two older Bamako photographers, Mountaga Dembelé and the French colonial resident Pierre Garnier.
Trypomastigotes were collected in the extracellular medium from five days onward.
To determine the effect of pUC/pU2 bicistronic constructs on angiogenesis of conditioned medium from meningioma cells, we performed vessel formation assay in human microvascular endothelial cells (Fig. 1D).
Fig. 4A shows a representative magnitude and time course of Lp response to B31-A3 spent medium from one of the vessels.
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