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The conditioned medium from these cells was then transferred to untreated cells and incubated for 72 h.
The gradual release of Ca, P, Si, Mg, Zn and Sr into the culture medium from these scaffolds contributed to the enhancement of the proliferation and ALP activity of mesenchymal stem cells (MSCs).
Medium from these transfected cells was collected 48 hrs after transfection and was filtered.
The conditioned medium from these cells, however, showed robust accumulation of the shed GOLIM4 ectodomain that depended upon β-secretase activity (Figure 3B, right panel).
Examination of the conditioned medium from these cells revealed a low level of shedding of Sema4C, which was greatly enhanced by increasing β-secretase activity (Figure 5A, right panel).
Therefore, we collected cell-free medium from these cells (ICN medium) maintained at 41°C (v-Src inactive) and from cells transferred to 37°C (v-Src active) for 72 hours, and examined its capacity to activate phosphorylation of the TGF-β signaling effector Smad2 [17], in QNR/v-srcts cells.
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Conditioned medium from the senescent fibroblasts recapitulated these findings, indicating that a large component of the growth-promoting effect was due to paracrine-acting factors.
What they brought to the venture was an eagerness to build a new medium from the bottom up.
The Seventh will receive instruction on its new medium from the 62nd Coast Artillery, Anti-Aircraft, now stationed at Fort Totten.
Endothelial cells were purchased from Cambrex and passaged in EGM2-MV medium from the same manufacturer.
Derivation and propagation of diploid mouse TS cells strictly relies on the stimulation with FGF4 and conditioned medium from MEF, as these conditions allow the proliferation and inhibit the differentiation of Cdx-2 - positive mouse TS cells to other types of trophoblast cells [4] [6].
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