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Burlaca's research showed that conditioned medium from endothelial progenitor cells promotes endothelial cell proliferation only after cell adhesion is induced by conditioned medium from mesenchymal stem cells.
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VSMCs were treated with conditioned medium (CM) from endothelial cells exposed to control or apelin siRNA for 48 h.
It has been shown that conditioned medium collected from endothelial cells stimulate phosphorylation of STAT3, Akt, and ERK in head and neck squamous cell carcinomas [ 26].
Conditioned medium from mature endothelial cells (HUVEC-CM) was obtained in parallel and applying the same protocol, respectively.
Visual inspection using a light microscope showed apparent cellular damage, therefore levels of LDH in the luminal (endothelial) medium from NADA-treated inserts were measured and were found to be significantly greater than vehicle at 48 h (P < 0.001, Figure 5F).
Conditioned medium from PGF2α-treated cells increased endothelial cell proliferation and branching (P < 0.05).
It was also shown that conditioned medium from heparanase-overexpressing cells sustain endothelial cell proliferation.
Conditioned medium from primary human dermal microvascular endothelial cells (HDMEC) stably transduced with silencing RNA for IL-6 (or controls) was used to evaluate the role of endothelial-derived IL-6 on the activation of key signaling pathways in tumor cells.
These results show that B31-A3 spent medium increased endothelial [Ca2+]i, while spent medium from B31-A did not have an effect.
In accordance with this, we found that immunoneutralisation of ADAMTS1 from conditioned medium from PGF2α-treated FPS cells enhanced endothelial cell proliferation compared with conditioned medium alone, indicating that ADAMTS1 is an inhibitor of endothelial cell proliferation.
HUVECs were cultivated in enhanced endothelial cell growth medium from PELOBiotech (Planegg, Germany) at 37 °C and 5.0%% CO2.
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