Sentence examples for medium from cultured from inspiring English sources

After incubation, the culture medium from cultured OA chondrocytes was collected and the PGE2, LTB4 and TGF-β1 levels were measured with specific commercial kits from Cayman Chemical and R&D Systems (Minneapolis, MN, USA), according to the manufacturer's instructions.

Growth-promoting effects on prostate cancer epithelial cells have been demonstrated with conditioned medium from cultured osteoblasts 54– 56 and in three-dimensional culture systems.

(F) the protein level of TGF-β was detected in conditional medium from cultured kidney macrophages of fibrotic kidney of RBP-J cKO and control mice by ELISA (n = 3).

The effect of spent medium from cultured B31-A3 cells on microvessel permeability was investigated in seven individual venular microvessels.

Here, to identify miRNA related to tumorigenesis and metastasis, we performed extensive miRNA analysis in three cellular fractions including cells, exosomes, and medium from cultured cells.

Medium from cultured microglia contained IFNγ and decreased the viability of NPCs, whilst blocking with anti-IFNγ antibodies counteracted this effect.

The culture medium from the cultured HACs at day seven was collected and kept frozen until the concentration of Prostaglandins E2 (PGE2) was measured with Parameter™ PGE2 Immunoassay Kit (R&D Systems Inc , USA.

The concentration of eCG in medium recovered from cultured chorionic girdle cells after 3 days in culture was determined using a pregnant mare serum gonadotropin enzyme linked immunoassay (DRG International), as previously described (44).

The human cytokine antibody array I (RayBiotech, Inc., Norcross, GA) was used according to the manufacturer's instructions using conditioned medium collected from cultured Ishikawa FPS cells treated with vehicle or 100 nM PGF2α for 24 h [9].

Briefly, the membranes were incubated with 2 ml blocking buffer for 30 min, followed by 2 h incubation with 1 ml of conditioned medium collected from cultured Ishikawa PROKR1 cells treated with or without 40 nM PROK1 for 8 h.

When BMSCs were stimulated by conditioned medium from macrophages cultured on Mg-β-TCP scaffolds, osteogenic differentiation of BMSCs was significantly enhanced; whereas osteoclastogenesis was inhibited, as indicated by the downregualtion of MCSF, TRAP and inhibition of the RANKL/RANK system.