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To perform an in vitro glucose tolerance test, medium from both culture compartments was completely removed and replaced with 315 µl medium, respectively, containing 11 mM glucose.
Comparison of serum-free conditioned medium from both cell lines showed conspicuous differences in the levels of several proteins, including a quartet of high molecular weight proteins (≥140 kDa).
M. rubrum survival was not affected in contact with purified OA, DTX-1 and PTX-2 solutions, but decreased significantly when the ciliate was exposed to cell-free or filtered culture medium from both D. cf. ovum and D. caudata, the latter containing moderate concentrations of free eicosapentaenoic acid and docosahexaenoic acid.
Semi-dynamic CAs adapt fast to the changes in the traffic pattern and the interference on the wireless medium from both internal and external sources.
Smooth muscle cells were then cultured in conditioned medium from both active and mutant caspase-1 transfected cells.
To investigate whether Bb-induced inflammation is associated with virulence, the effects of spent medium from both virulent (B31-A3) avirulentlent (B31-A) strains on microvessel Lp and endothelial [Ca2+]i were investigated.
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Decorin and MIP-1γ were not detectable in the conditioned medium from MB49, whereas both were readily quantified in the conditioned medium from MB49-I, thus confirming the differential expression evidenced by the antibody array experiment (Fig 4B).
Western analysis of medium samples from both the scGlaGus or mcGlaGus strains failed to detect the GlaGus protein in the medium, using Gus antiserum.
Liquid chromatography-mass spectrometry was used to globally characterize the secretome, collected as conditioned medium (CM), from both MDC and AFDC.
Co-culture experiments and treatment with conditioned medium harvested from both types of osteoblasts were performed to investigate potential interactions between the two different tissue origin osteoblasts.
To identify factors selectively secreted by G2B-10A oR2-T1ASAS cells, we screened conditioned medium derived from both cell cultures, using a cytokine antibody array.
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