Sentence examples for medium fraction of from inspiring English sources

Immunoblotting experiments on the cell and medium fraction of transfected HEK293-T cells demonstrated that, analogous to the wild type protein, both aberrant proteins were produced and efficiently secreted into the extracellular matrix (Figure 4D).

Using gel-permeation chromatography in combination with light scattering measurements (see Viebke et al. [21]), it was confirmed that the low fraction had an average molecular weight of 10 kDa, and the medium fraction of 40 kDa.

For both stimuli, a medium fraction of all cells undergoes apoptosis (compared to the inhibition of antioxidants and 500 μM TLC-S, see below).

They are released into the medium fraction of the lgt-mutant because of their inefficient anchoring to the membrane (Fig 2B).

As shown by Western blotting using polyclonal antibodies against PknB, the full-size PknB was detectable both in the cellular and growth medium fractions of cells of S. aureus NCTC 8325 harvested at an OD600 of 2. As expected, PknB was neither detectable in cellular nor growth medium fractions of the ΔpknB mutant.

The medium fraction <3 kDa of control conditions and K. oxytoca-exposed cells was concentrated (10×) by lyophilisation followed by dissolving in loading buffer [200 mM Tris HCl (pH 6.8), 2% SDS, 40% glycerol, 0.04% Coomassie brilliant blue, 2% β-mercapto-ethanol].

For MALDI-TOF analysis of the conditioned medium fraction <3 kDa, samples of control-conditions and K. oxytoca-exposed cells (<3 kDa) were treated with SPE STRATA X (Phenomex, Utrecht, The Netherlands).

The other half of HSPC/MSC medium fraction was incubated with goat anti-mouse IgG-magnetic-beads as an additional control.

Half of the HSPC/MSC medium fraction and the control fraction were then incubated each with mouse mAb CD133-magnetic-beads (Miltenyi Biotec).

Briefly, the medium fraction was obtained by an additional centrifugation of 1 ml supernatant (10 min/16,530-g/4°C min/16,530-g/4°C

To ensure comparability between culture supernatant fraction and cell pellet fraction, the pellet from cell supernatant fraction (medium fraction, M) were resuspended in the same volume of PBS buffer as that used for cell pellet (cellular fraction, C).