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Anhydrite-bearing grains are only observed in the medium fraction.
††: Samples named "coarse" is a grain in the coarse fraction and "med" is a grain in the medium fraction.
c Grain of altered rock that belongs to the silica K-feldspar ± albite ± garnet ± biotite asilica K-feldsparmedium fraction.
Briefly, recombinant cultures (0.8 mL) at various induction time points were taken and centrifuged to separate into medium (medium fraction, M) and cell pellets (cellular fraction, C).
There is no mineralogical difference between the coarse and medium fractions except for the presence of calcium sulfate minerals in the medium fraction.
For MALDI-TOF analysis of the conditioned medium fraction <3 kDa, samples of control-conditions and K. oxytoca-exposed cells (<3 kDa) were treated with SPE STRATA X (Phenomex, Utrecht, The Netherlands).
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We then expressed this protein in NIH3T3 cells, fractionated the cells into nuclear, cytoplasmic and extracellular (culture medium) fractions and analyzed these by immunoblotting using an anti-HA antibody.
The combined medium fractions represent the amount of free radioligand.
Polished sections were prepared for the SEM EDS observation on the coarse and medium fractions.
Feldspar and anhydrite only occur in the course and the medium fractions.
Lipids from the cell homogenate and culture medium fractions were analyzed by HPTLC with 5 4 1 CHCl3-MeOH-0.25% aq KCl as developing solvent.
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