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For lysosomal co-localization, harvested DCs were cultured overnight in glass-bottom culture dishes (Mattek™), incubated with 2 nM QD-containing medium for varying times at 37°C.
Harvested DCs were cultured overnight in glass-bottom culture dishes (Mattek™), incubated with 2 nM QD-containing medium for varying times at 37°C, and processed as described [23].
The cells on the ALI were pre-treated with AR inhibitor, fidarestat (10 µM), for over-night from the basal side in differentiation medium without EGF beginning day 11 after establishment of ALI and stimulated with IL-13 by addition of recombinant human IL-13 (25 ng/ml) to the medium for varying time periods as indicated.
The cells were washed in PBS and re-fed with complete medium for varying release times before UVC or mitomycin C treatment.
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Prior to infection with A. rhizogenes, stem sections with one node were pre-cultured in MS medium for a varying period from 0 to 6 days, after which the standard procedure described in Methods was used for the remaining part of the assay.
While some studies have been published on the transport of ZnO nanoparticles in individual porous media, these studies do not generally account for varying porous medium composition both within and between aquifers.
The stained cells were washed, and then incubated for varying durations in culture medium at 37°C.
This work demonstrates the potential of medium engineering as a powerful additional tool for varying enzyme selectivity and thus engineering the product range of biotransformations.
After treatment at low glucose concentrations (3 mM) for 16 (MIN6 β-cells) or 1 h (islets), we further incubated islets (Fig. 1A) or MIN6 β-cells (Fig. 1B) in medium containing 16.7 or 30 mM glucose, respectively, for varying times.
PCa cells were treated with vitamin-E isomers for 24- and 48-h at increasing dosage (low: 20 μ M, medium: 40 μ M and high: 80 μ M) and for varying time points.
Plates were incubated at 37°C for 3 days, after which the EBs were flushed with differentiation medium and incubated in 24-well or 10-cm plates for varying periods of time.
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