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Do not use full nutrient for top-ups.
After rocking at room temperature for 1 h, the inoculum was aspirated, and the appropriate volume of infection medium was added to top up each well of the plate.
Medium was topped up to 0.5 ml after 1 day, then replaced every 3 days.
After 24 h, the medium was topped up to 1 ml.
We have to look for some extra money to top up our low salaries".
Draw off Dispase II from the other well and transfer to a sterile 15 ml tube and top up with complete medium.
(Top up as necessary).
Instead, it said, "The Internet is a grown-up medium for grown-up-type activities".
In the following study, the complex was prepared in 100 μL medium without serum and then topped up to 1 mL with DMEM without serum for transfection purposes.
The following day flasks were topped up with osteoinductive medium, (basal medium supplemented with 0.25 mM ascorbic acid, 10 mM β-glycerophosphate and 100 nM dexamethasone (all Sigma-Aldrich)).
Pay for the top-up.
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