Finally, GMSCs encapsulated in the photopolymerized gel maintained their viability when cultured in osteogenic medium for three weeks.
After being subcultured in the LD4 medium for three intervals of eight-weeks, plantlets had bulb circumferences of 1 2 cm with 4 6 leaves.
After 2 weeks, the HAT medium was replaced with HT medium for three passages followed by returning to the normal cell culture medium.
Genomic DNA was extracted from cultures grown on 40 g/L glucose, 5 g/L peptone, 5 g/L yeast autolysate, and 20 g/L agar (GPYA) medium for three days using the FastDNA kit (BIO101, Carlsbad, CA, U.S.A).
Finally, computational fluid dynamics (CFD) simulations are performed to determine the effect of light reflection, refraction and absorption at the air quartz water interfaces on incident radiation entering the reaction medium, for three quartz tube radius to lamp radius ratios (rquartz/rlamp) and two typical quartz tube thicknesses.
They were then incubated in fresh medium for three hours.
The magnetically enriched cells (over 80% pigmented late stage IRBCs) were incubated on the PHA-E coated coverslip for 10 min before free and loosely attached cells were gently washed by rinsing in medium for three times.
Chondrogenic differentiation was achieved through culturing of a pellet of 2.2×105 cells in differentiation medium for three weeks with subsequent assessment of acid mucopolysaccharides by 1% Alcian blue (Chroma, Köngen, Germany) for 10 30 min. [54].
The TCII-OLEO transfected cells had a dramatically higher binding with labeled B12 in the membrane fraction, compared with other transfected cells, when 57Co-labeled Cobalamin was incorporated into culture medium for three days (Fig. 1C).
