Sentence examples for medium for selection from inspiring English sources

The transformants were spread on SPASC medium for selection, and several transformants were selected, based on colony size.

After transfection according to the methods mentioned above, 20 µg/ml puromycin was added into medium for selection.

For stable selection of iASPP-depleted cells, 0.5  μg/ml puromycin was added to the medium for selection.

For marker-assisted transformation, 100 mg/ml Kanamycin was added to ZCVK medium and CK medium for selection of transgenic shoots.

Forty-eight hours after the second infection, 350  μg ml−1 of hygromycin was added to the culture medium for selection of infected cells.

After 5 days, the cells were split 1 : 3, and 1 mg/mL G418 was added to the medium for selection for 10 days; the medium was replaced with fresh medium every 2 to 3 days.

The kanamycin-resistant calluses were then cultured on the fresh selection medium for further selection and multiplication.

Six non-randomised controlled cohort studies (16 018 women) were included in the review (table 1[t1] and fig 1). 1 3 8 10 11 12 When methodological quality was assessed on the Newcastle-Ottawa scale, most studies had a medium risk for selection bias and medium to high risk for comparability and outcome assessment (table 2[t2]).

Following transfection, HK293 cells were grown in 5% CO2 to 80% confluence in DMEM supplemented with 4.5 mM glucose, 10 mM HEPES, 10% fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin and geneticin (700 μg/ml medium) for the selection of geneticin-resistant clones, with selection medium changed every 3 4 days.

Every 3-4 weeks, transformants were selected and transferred onto fresh medium for continued selection.

The transformants were plated onto SD/-Trp medium for positive selection.