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TSC2−/−ASM cells require epidermal growth factor (EGF) in the growth medium for proliferation, and its replacement with IGF-I greatly reduces cell growth.
Using a low serum medium for proliferation, we clarified the role that DA plays in promoting proliferation during 7 days of culture.
Myogenic cells were grown on gelatin-coated dishes in high serum growth medium for proliferation then switched to low serum differentiation medium to induce myotube formation [ 33, 45].
The human primary myogenic cells were grown on gelatin-coated dishes in high-serum medium for proliferation and switched when near confluence to low-serum medium for differentiation as described.
The mouse C2C12 muscle cell line (a myoblast cell line maintained in growth medium for proliferation that, upon transfer to an appropriate stimulant medium, differentiates into myofibres), and in vitro differentiation have been used in a number of studies [ 7, 13, 32].
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The Internet has been an ideal medium for the proliferation of promotional blather, especially among nonexperts.
This supports the fact that alginate is a suitable medium for cell proliferation (Loty et al. 1998).
Introduction of hardware to appropriately manage a fracture becomes yet another suitable medium for bacterial proliferation given that most metals are electrochemically active and promote molecular adhesion [5].
All samples were placed in quadruplicate and also were made four wells with medium with DMSO (1.5 μl) as negative control and control wells (blank wells or standard) with normal medium for cell proliferation and wells with only medium without cells.
Primary endothelial cells remained in terminal growth arrest and could not be rescued by mitogenic growth medium for further proliferation.
HUVECs were incubated in endothelial basal medium mixed with the same volume of 5-fold concentrated medium for cell proliferation, migration, and tube formation in vitro.
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