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The 20 or 100 mL mineral salt medium for main culture (in 100 and 500 mL baffled shake flasks, respectively) were inoculated with a resulting OD600 between 0.05 and 0.1.
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These extensive screening studies were carried out in distilled water (instead of using phosphate buffered saline or cell culture medium) for two main reasons.
English remains the main medium for administration, commerce, and industry, and it is the primary language of instruction in schools.
Speech is the main medium for human communication and interaction.
Some predicted that electronic books would become the main medium for reading by 2015.
Normally television advertising, the main medium for reaching the electorate, would help.
LISA NAPOLI PROMOTING LIVE PERFORMANCE -- When the impresario P.T. Barnum brings the Swedish singer Jenny Lind to the United States for a 93-city tour in 1850, live performance remains the main medium for popular music.
What's more, the stated gross profit margin of 59% excludes the cost of TV advertising, which doesn't come cheap and is Purplebricks' main medium for building its brand.
With a record number of observers at polling stations, election day was a fever of accusations of fixed votes on the social media outlets that are the main medium for the opposition.
Although window is the main medium for the lighting of the building, it contributes significantly in the building's energy consumption, either commercial or residential.
The photobook, rather than the gallery, was the main medium for this psychic exploration and it is good to see Kikuji Kawada's extraordinary undertaking, The Map – perhaps the most intricately designed and powerfully evocative Japanese photobook ever – on display alongside his prints.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com