Sentence examples for medium for genomic from inspiring English sources

Colonies resistant to 5-FOA were cultured in nonselective complex medium for genomic DNA isolation and subsequent PCR screening of the targeted region using primers JF204 and JF049.

Single colonies were then picked to liquid MYG medium for genomic DNA isolation using the Wizard Genomic DNA Purification Kit (Promega, Madison, WI).

The transformants were inoculated into liquid medium for genomic DNA extraction and subsequent PCR screening of the target region to confirm the homologous recombination event using two primer sets of DC262/DC410 and DC230/DC411.

Cells were then plated onto solid LOD medium and uracil prototrophic transformants were inoculated into liquid medium for genomic DNA extraction and subsequent PCR screening of the targeted region of the chromosome.

Cells were then plated onto solid defined medium (without uracil and casein) and uracil prototrophic transformant colonies were inoculated into liquid medium for genomic DNA extraction and subsequent PCR screening of the targeted region.

Uracil prototrophic transformants were selected and used to inoculate into liquid medium for subsequent genomic DNA extraction and confirmation of the pDCW180 knock-in into the targeted chromosome via PCR screening.

Uracil prototrophic transformants were selected and used to inoculate into liquid medium for subsequent genomic DNA extraction and confirmation of the pDCW171 knock-in into the chromosome via PCR screening.

Colonies resistant to 5-FOA were cultured in medium containing uracil for genomic DNA isolation and PCR screening of the targeted region.

For the screen of vegetatively growing cells, the mutant pool pre-grown in YES medium was treated with 500 Gy of ionizing radiation (IR) using a Cs Gammacell 1000 irradiator (dose rate 16 Gy/min), grown for five OD600 doublings in YES medium, and then harvested for genomic DNA preparation.

Out of 100 T2 YoeBSpn-GFP transgenic plants that grew on the selection medium, 11 were randomly chosen for genomic DNA preparation and PCR amplification using GFP-specific primers.

For aligning medium length genomic sequence reads (150-500 bagainstgainst related genomes, it is not immediately clear which program and which parameters would yield the best compromise between sensitivity and specificity.