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The composition was modified from the Czapek-Dox, a generally used synthetic minimal medium for fungi (0.2% NaNO3, 0.1% K2HPO4, 0.05% MgSO4·7H2O, 0.05% KCl, 0.001% FeSO4·7H2O), with 1% emulsified PBSA (Bionolle EM-301) as a sole carbon source instead of sucrose.
The spoiled eggs are an excellent culture medium for fungi, especially Saprolegnia.
They showed differences between the mean time to positivity for Candida albicans between the specific culture medium for fungi versus a non-specific medium for fungi (aerobic culture), 34 ± 25 h vs. 42 ± 19 h, respectively [ 23].
It should be noted that in our study we did not use specific culture medium for fungi.
After five days of culture, PBS was decanted into a special glass tube containing medium for fungi (Sabouraud 2 agar (SAB2-T), BioMerieux, Marcy l'Etoile, France).
However another study also used specific culture medium for fungi (BACTECTM Myco/F Lytic) whose time to positivity is known to be shorter, especially in the identification of non-albicans Candida species.
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What may be the best medium for one fungus could often prove unproductive for other strains.
In order to determine the MFC, an aliquot of 10 µL of each incubated well of MIC and the higher concentrations were sub-cultured on Petri dishes containing specific solid medium for each fungus and incubated at 25 °C for 5 days.
Towels or clothes may provide a medium for contracting fungus.
Towels or clothes may provide a medium for contracting fungus by sticking in the fabric.
This eliminates the best medium for promoting fungus growth – damp skin.
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