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The collected specimen was delivered on ice to the Tissue Bank, where it was divided into three parts: one in OCT embedding medium for frozen tissue specimens (Tissue-Tekâ) and two directly frozen in liquid nitrogen.
Tissue-Tek OCT Compound (embedding medium for frozen tissue specimens) was procured from Sakura (Torrance, CA).
Animals were sacrificed at 8 months and their brains were embedded in OCT medium for frozen sectioning onto glass slides.
Remove brains from the sucrose and embed in an embedding medium for frozen tissue specimens such as Tissue-Tek OCT (Sakura Tokyo, Japan).
Cryopreserved samples were embedded in medium for frozen tissue specimens (Tissue-Tek OCT; Sakura Finetek, Torrance, Calif., USA) and fitted into a cryostat (CM1850 UV; Leica Microsystems, Nussloch, Germany) for histological analysis.
The ileal tissue was gently washed with normal saline at room temperature, cut into 5 × 5 mm sections, and placed in Cryomatrix (Thermo Scientific, Pittsburgh, PA) medium for frozen sectioning.
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Another significant use is as a protective medium for freezing red blood cells, sperm cells, eye corneas, and other living tissues.
Cultures were grown to an OD of 0.8 to 1.2, pelleted and resuspended in 50 ml medium for freezing.
Embryonic heads (E13.5 or younger), dissected brains (E14.5-P1 E14.5-P1nscardially perfused brains fror adultranscardiallye fixed in 4% formalin in perfusede-brainsed saline (PBS) at 4°C overnight, equilibrated in 30% sucrose and embedded in Tfromissue frozen medium (TBS) for frozen sectioning.
The cell spheres were subsequently embedded in OCT medium (Sakura Finetek) for frozen sections.
In continuation of this systematic and field-wide effort to harmonize ELISPOT assay, CIP in cooperation with CIC organized a proficiency panel to test the impact of serum in the medium used for freezing cells prior to the assay.
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