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Surfaces were mounted using mounting medium for fluorescence, with DAPI counterstain (Vector Laboratories), and viewed by fluorescent microscopy (Zeiss Axiophot).
Slides were mounted in medium for fluorescence with DAPI for nuclear counterstaining (Vector Laboratories) and observed in Zeiss Axiovert microscope.
UltraCruz® mounting medium for fluorescence studies with DAPI was supplied by Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).
Sections were then incubated with the primary antibodies for 24 hours at 4°C, washed in PBS, incubated for one hour at RT with the fluorescent secondary antibodies, washed in PBS again and mounted using Vectashield® mounting medium for fluorescence with DAPI Vectro Laboratoriess, USA).
Samples were subsequently mounted with aqueous medium for fluorescence (Sigma).
The sections were mounted in Vectasheild Mounting medium for Fluorescence (Vector Laboratories, CA 94010).
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The slides were air dried and mounted with an aqueous mounting medium made for fluorescence (Gel Mount), with DAPI (4′,6-diamidino-2-phenylindole) (VectaShield HL1200).
We compared the protein profiles in cells treated with TXL/PA, PA, ethanol and control medium for the fluorescence intensity as illustrated in the Additional file 2.
The slides were mounted with a mounting medium containing DAPI for fluorescence (Vectashiedl; Vector Laboratories, Burlingame, CA).
For microscopy, cells of the 15,000 clones were mixed, grown in complex medium, and analyzed for fluorescence.
The slides were then mounted with vectashield hardest mounting medium with DAPI for fluorescence (Vector laboratories, Peterborough, United Kingdom).
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