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Dupont and Schwarz obtained a fresh ovary from a woman who was the victim of an aeroplane accident, and kept it living in their medium for five years.
Per genotype, twelve animals were inoculated, kept in pairs in 2.5 ml of artificial medium for five days and fed daily 3×105 algae cells.
Cells forming EBs were cultured in EB medium for five days, before positive selection based on secretion of human chorionic gonadotrophin (hCG) and transferral to adherent plates containing MEFs.
Cells were grown in ganciclovir-containing medium for five or more days, with subculturing performed when necessary.
After two days, medium was changed to eliminate non adherent cells and cultivated in fresh medium for five days.
Currently, expression levels are available from samples of germinating Ashbya somatic spores incubated in rich medium for five, seven and nine hours (panel b).
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A small pumpkin went for a buck, medium for two.
It's the perfect medium for two cultures to come together".
Cells were plated in differentiation medium for four hours and then monitored.
"It'll be much more exciting than working in one medium for three years," she says.
The cells were maintained in this medium for three days until spheroid formation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com